Research areas and outputs.
Germ-layer cells obtained through different paths exhibit distinct transcriptional profiles during differentiation. Based on such observations, we want to investigate whether functional cells obtained via different induction routes with different have different functions and therapeutic potential in the context of the endodermal lineage.
This study aims to develop a chemically defined method for obtaining mesenchymal stem cells (MSCs) from pluripotent stem cells (PSCs) through different germ layers and investigates the key components in the traditional serum culture medium that are essential for in vitro induction of MSCs.
Human PSC colonies exhibit a unique epithelial-mesenchymal gradient of morphology from the periphery to the centre, which reflects a complex interplay of the microenvironment, signalling pathways, cell adhesion, and mechanisms. We aim to employ finite element analysis to establish an in silico platform that can overcome the limitations of traditionally used and technically challenged traction force microscopy, in order to investigate the mechanical properties of pluripotent stem cell colonies and understand the reasons for such interesting morphology.
Additive manufacturing offers great potential to fabricate more effective prostheses used for total knee arthroplasty (TKA). While porous implants could provide better osseointegration and avoid stress shielding, a porous structure could compromise the mechanical strength of these prostheses. Therefore, it is necessary to optimize the design of these structures to achieve a balance between osseointegration and mechanical functions. In this project, finite element analysis (FEA) will be employed to investigate the correlation between peak stress and pore size in TKA prostheses made of Biolox® delta ceramic and tantalum, thereby optimizing the design of these prostheses for enhanced strength and osseointegration.
This study explores hydrogels, a type of biomaterial with excellent biocompatibility and easy preparation, as potential carriers for cell therapy and 3D cell culture scaffolds. We used a hydrogel made from dibenzaldehyde-terminated poly (ethylene-glycol) (DF-PEG) and glycol chitosan (GC), which is self-healing, injectable, and quickly prepared. The hydrogel’s adaptability as a 3D cell culture medium was tested by adjusting its mechanical strength to find ideal conditions for different tissue cells. This research contributes to the development of universal theories for 3D culture based on hydrogels.